Measurement of Hyperpolarized 15 N - choline using Polarization Transfer with 1 H Detection

نویسندگان

  • S. Jannin
  • J. van der Klink
  • R. Sarkar
  • G. Bodenhausen
  • D. Kirik
چکیده

pulse sequence, applied ca 110 s after dissolution of the hyperpolarized N-choline sample. Figure 1. Top: The H spectrum resulting from the non-refocused INEPT pulse sequence, applied ca 35 s after dissolution of the hyperpolarized N-choline sample. Bottom: a thermal-equilibrium scan using the same sequence (magnified). Measurement of Hyperpolarized N-choline using Polarization Transfer with H Detection

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Proton NMR of (15)N-choline metabolites enhanced by dynamic nuclear polarization.

Chemical shifts of protons can report on metabolic transformations such as the conversion of choline to phosphocholine. To follow such processes in vivo, magnetization can be enhanced by dynamic nuclear polarization (DNP). We have hyperpolarized in this manner nitrogen-15 spins in (15)N-labeled choline up to 3.3% by irradiating the 94 GHz electron spin resonance of admixed TEMPO nitroxide radic...

متن کامل

Indirect Detection of Enzymatic Processes by Hyperpolarized NMR: Temporal Information, Enhanced Spectral Resolution and Slow Spin Relaxation

Introduction Continuous efforts are invested in developing improved NMR techniques to characterize metabolism. Particularly pressing is the need to enhance NMR’s sensitivity. The last years have witnessed what promises to become a major development in this area, with the advent of ex situ dynamic nuclear polarization (DNP). The long transfer times needed by this ex situ approach imply that only...

متن کامل

Dynamic 1H imaging of hyperpolarized [1‐13C]lactate in vivo using a reverse INEPT experiment

PURPOSE Dynamic magnetic resonance spectroscopic imaging of hyperpolarized 13 C-labeled cell substrates has enabled the investigation of tissue metabolism in vivo. Currently observation of these hyperpolarized substrates is limited mainly to 13 C detection. We describe here an imaging pulse sequence that enables proton observation by using polarization transfer from the hyperpolarized 13 C nucl...

متن کامل

Over 20% 15N Hyperpolarization in Under One Minute for Metronidazole, an Antibiotic and Hypoxia Probe

Direct NMR hyperpolarization of naturally abundant (15)N sites in metronidazole is demonstrated using SABRE-SHEATH (Signal Amplification by Reversible Exchange in SHield Enables Alignment Transfer to Heteronuclei). In only a few tens of seconds, nuclear spin polarization P(15)N of up to ∼24% is achieved using parahydrogen with 80% para fraction corresponding to P(15)N ≈ 32% if ∼100% parahydroge...

متن کامل

Kinetic analysis of hyperpolarized data with minimum a priori knowledge: Hybrid maximum entropy and nonlinear least squares method (MEM/NLS).

PURPOSE To assess the feasibility of using a hybrid Maximum-Entropy/Nonlinear Least Squares (MEM/NLS) method for analyzing the kinetics of hyperpolarized dynamic data with minimum a priori knowledge. THEORY AND METHODS A continuous distribution of rates obtained through the Laplace inversion of the data is used as a constraint on the NLS fitting to derive a discrete spectrum of rates. Perform...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

عنوان ژورنال:

دوره   شماره 

صفحات  -

تاریخ انتشار 2008